Kezia Dugdale has called on Nicola Sturgeon to keep SNP promises on protecting local NHS services made before the Holyrood election.
The Scottish Labour leader increased pressure on the First Minister to step in over proposals to downgrade or close services in some areas.
On Wednesday, the minority Scottish Government suffered a parliamentary defeat after opposition parties united behind a Labour motion urging Health Secretary Shona Robison to ''call-in'' the proposals.
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Labour argues the Government must designate plans from NHS Greater Glasgow and Clyde, NHS Lanarkshire and NHS Lothian as ''major service changes'', meaning they would require ministerial approval before they could go ahead.
Speaking during First Minister's Questions at Holyrood, Ms Dugdale said those at risk include maternity services at the Vale of Leven Hospital in Alexandria, Glasgow's Lightburn Hospital, and cleft palate services at the Royal Hospital for Sick Children in Edinburgh.
She said: "On the steps of Bute House the day after May's election, the First Minister said this, 'We will always respect the people, now and in the future. We simply ask that other parties do likewise'.
"Last night this Parliament, the representatives of the people of Scotland, spoke with one voice on the planned cuts to local NHS services, so will the First Minister now respect the will of this Parliament?"
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Ms Dugdale said that while her party recognises there is a due process, it is "angry" that during the election SNP candidates and Ms Sturgeon had "promised people that these services were safe when they were not".
She continued: "The truth is that as a Parliament we agreed that the Health Secretary should call-in proposed cuts by health boards in Glasgow, Lanarkshire and the Lothians, and that's because these services are vital to communities across the country - but they are now at risk.
"Now that the Parliament has spoken, will she immediately call these decisions in and reject the planned cuts?"
She added: "This Parliament has now said that promises made before the election must be delivered on.
"If the vote of this Parliament, elected by the people of Scotland, doesn't make the First Minister keep her promises, just what will?"
Ms Sturgeon insisted no decisions have been taken on any of the proposed changes and hit out at what she described as the "complete incoherence at the heart of Scottish Labour" as she emphasised there is a due process to follow.
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The First Minister said: "There is a well-established and long-standing process in place to consider proposals for service change.
"The early stages of that process, which Greater Glasgow and Clyde health board are currently engaged in, inform the judgement about whether or not a particular service change is to be considered major and therefore ultimately decided by ministers.
"When the process, the long-established process, has reached the stage where that judgement can be made, the Health Secretary will report it to Parliament and in reaching that judgement of course she will take account of the debate and the decision of the Scottish Parliament yesterday."
Hitting back at Ms Dugdale, she added: "Week in, week out we have the Labour Party standing up in this chamber accusing this Government of undermining local decision making.
"Today we have them standing up and demanding that we undermine local decision making. It is an absolute shambles.
"We will take no lessons from Labour when it comes to standing up for local health service because, unlike Labour, we have demonstrated as a Government, time and again, our willingness to actually block changes when they are not in the interest of patients."
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